ec35 backbone (New England Biolabs)

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New England Biolabs ec35 backbone
Ec35 Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13738 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bamhi/BamHI/pm42834167-436-8-17
Average 99 stars, based on 13738 article reviews

ec35 backbone - by Bioz Stars, 2026-10

99/100 stars

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FLAG-tag:

Article Title: A Novel Dominant-negative CARD11 Variant Causes Atopy and Immunodeficiency Syndrome
Article Snippet: .. The 3x FLAG tag was removed from the parental CARD11-3x FLAG construct via restriction digest with BamHI and NheI, and the V5 epitope tag was inserted using NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs) according to the manufacturer’s instructions. .. Successful generation of the mutated plasmid and insertion of the V5 epitope tag were verified via Sanger sequencing, and all plasmids were isolated for transfection from transformed NEB AR TIC LE IN PR ES S ARTICLE IN PRESS ACCEPTED MANUSCRIPT 5-alpha competent Escherichia coli (New England BioLabs) using a GenElute HP Plasmid Maxi-Prep kit (Sigma-Aldrich).

Construct:

Restriction Assay:

Isolation:

Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia.
Article Snippet: .. To generate pLJM1-Smc3, pLJM1 (Addgene #19319) was digested with NheI (NEB R3131) and BamHI (NEB R3136) and the large fragment (hereafter, ‘backbone’) isolated by agarose gel electrophoresis followed by extraction and purification (QIAquick Gel Extraction Kit #28704). .. Using pCAGGS-3XFLAG-Smc3-mKate2-bpA (Addgene #156447) as template, Smc3 was amplified and homology arms compatible with Gibson Assembly were introduced by PCR (NEB #M0492).

Agarose Gel Electrophoresis:

Extraction:

Purification:

Gel Extraction:

Recombinant:

Article Title: Molecular Characterization of OsHAD Phosphoprotein: Expression, Catalytic Properties, and Functional Annotation.
Article Snippet: Phosphoproteins and phosphatases play crucial roles in regulating diverse cellular processes in plants, including signal transduction, stress responses, and metabolic control, primarily through reversible phosphorylation.. However, the functional and biochemical information on many rice phosphatases remains limited.. In this study, we report the functional and biochemical characterization of OsHAD-2, a previously uncharacterized haloacid dehalogenase (HAD) family phosphatase from Oryza sativa.OsHAD-2 was heterologously expressed in Escherichia coli BL21 (DE3) and purified to homogeneity using GST-affinity chromatography.

Polymerase Chain Reaction:

Article Title: Disrupting SOX2 self-association and condensate formation to overcome chemotherapeutic drug resistance in lung squamous cell carcinoma
Article Snippet: .. The resulting PCR products were recombined into the pHAGE-3 × Flag vector, which was digested with BamHI (NEB, R3136V) and AgeI (NEB, R3552L) via homologous recombination (YEASEN, 10922ES20). .. Similarly, the 6 × His-tagged Helix 1–3, Flag-SOX2, and HA-SOX2 fragments were amplified and recombined into the pSin-Flag vector digested with BamHI and EcoRI (NEB, R3101V).

Plasmid Preparation:

Homologous Recombination:

other:

Article Title: Hemin activates interleukin-17 signaling and CCAAT/enhancer-binding protein beta to promote neuroinflammation and blood-brain barrier disruption.
Article Snippet: Heme overload, a feature of hemolytic conditions and cellular injury, can initiate oxidative stress and inflammatory cascades that perturb neurovascular homeostasis.. However, the molecular mechanisms by which peripheral heme exposure disrupts blood–brain barrier (BBB) integrity remain incompletely defined.. Here, we investigated key molecular mediators linking peripheral hemin challenge to neurovascular injury.

Review