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high fidelity restriction enzyme bamhi  (New England Biolabs)


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    Structured Review

    New England Biolabs high fidelity restriction enzyme bamhi
    High Fidelity Restriction Enzyme Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bamhi/BamHI/pmc13183001-150-4-8
    Average 99 stars, based on 13260 article reviews
    high fidelity restriction enzyme bamhi - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia.
    Article Snippet: .. To generate pLJM1-Smc3, pLJM1 (Addgene #19319) was digested with NheI (NEB R3131) and BamHI (NEB R3136) and the large fragment (hereafter, ‘backbone’) isolated by agarose gel electrophoresis followed by extraction and purification (QIAquick Gel Extraction Kit #28704). .. Using pCAGGS-3XFLAG-Smc3-mKate2-bpA (Addgene #156447) as template, Smc3 was amplified and homology arms compatible with Gibson Assembly were introduced by PCR (NEB #M0492).

    Agarose Gel Electrophoresis:

    Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia.
    Article Snippet: .. To generate pLJM1-Smc3, pLJM1 (Addgene #19319) was digested with NheI (NEB R3131) and BamHI (NEB R3136) and the large fragment (hereafter, ‘backbone’) isolated by agarose gel electrophoresis followed by extraction and purification (QIAquick Gel Extraction Kit #28704). .. Using pCAGGS-3XFLAG-Smc3-mKate2-bpA (Addgene #156447) as template, Smc3 was amplified and homology arms compatible with Gibson Assembly were introduced by PCR (NEB #M0492).

    Extraction:

    Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia.
    Article Snippet: .. To generate pLJM1-Smc3, pLJM1 (Addgene #19319) was digested with NheI (NEB R3131) and BamHI (NEB R3136) and the large fragment (hereafter, ‘backbone’) isolated by agarose gel electrophoresis followed by extraction and purification (QIAquick Gel Extraction Kit #28704). .. Using pCAGGS-3XFLAG-Smc3-mKate2-bpA (Addgene #156447) as template, Smc3 was amplified and homology arms compatible with Gibson Assembly were introduced by PCR (NEB #M0492).

    Purification:

    Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia.
    Article Snippet: .. To generate pLJM1-Smc3, pLJM1 (Addgene #19319) was digested with NheI (NEB R3131) and BamHI (NEB R3136) and the large fragment (hereafter, ‘backbone’) isolated by agarose gel electrophoresis followed by extraction and purification (QIAquick Gel Extraction Kit #28704). .. Using pCAGGS-3XFLAG-Smc3-mKate2-bpA (Addgene #156447) as template, Smc3 was amplified and homology arms compatible with Gibson Assembly were introduced by PCR (NEB #M0492).

    Gel Extraction:

    Article Title: Transcription and cohesin direct domain boundary spatial positioning and are linked to Friedreich's ataxia.
    Article Snippet: .. To generate pLJM1-Smc3, pLJM1 (Addgene #19319) was digested with NheI (NEB R3131) and BamHI (NEB R3136) and the large fragment (hereafter, ‘backbone’) isolated by agarose gel electrophoresis followed by extraction and purification (QIAquick Gel Extraction Kit #28704). .. Using pCAGGS-3XFLAG-Smc3-mKate2-bpA (Addgene #156447) as template, Smc3 was amplified and homology arms compatible with Gibson Assembly were introduced by PCR (NEB #M0492).

    Plasmid Preparation:

    Article Title: Arylsulfatase L is a Golgi chondroitin sulfatase regulating skeletal development.
    Article Snippet: The rat Arsl sequence was then cloned into the p3XFLAG-CMV-14 plasmid (#E7908, Sigma-Aldrich) using the following primers: FLAG – rat Arsl Fw: 5’ CCCAAGCTTGGGATGGCTCCGCCC 3’; FLAG – rat Arsl Rev:3’ CGGGATCCGCTTCCGGCTCCCGCC 5’. .. The vector was digested using HindIII and BamHI (NEB) restriction enzymes and treated with 5 μl of CIP (alkaline phosphatase, NEB), followed by ligation using the Quick Ligation Kit. ..

    Article Title: Disrupting SOX2 self-association and condensate formation to overcome chemotherapeutic drug resistance in lung squamous cell carcinoma
    Article Snippet: .. The resulting PCR products were recombined into the pHAGE-3 × Flag vector, which was digested with BamHI (NEB, R3136V) and AgeI (NEB, R3552L) via homologous recombination (YEASEN, 10922ES20). .. Similarly, the 6 × His-tagged Helix 1–3, Flag-SOX2, and HA-SOX2 fragments were amplified and recombined into the pSin-Flag vector digested with BamHI and EcoRI (NEB, R3101V).

    Article Title: Hemin activates interleukin-17 signaling and CCAAT/enhancer-binding protein beta to promote neuroinflammation and blood-brain barrier disruption.
    Article Snippet: Heme overload, a feature of hemolytic conditions and cellular injury, can initiate oxidative stress and inflammatory cascades that perturb neurovascular homeostasis.. However, the molecular mechanisms by which peripheral heme exposure disrupts blood–brain barrier (BBB) integrity remain incompletely defined.. Here, we investigated key molecular mediators linking peripheral hemin challenge to neurovascular injury.

    Ligation:

    Article Title: Arylsulfatase L is a Golgi chondroitin sulfatase regulating skeletal development.
    Article Snippet: The rat Arsl sequence was then cloned into the p3XFLAG-CMV-14 plasmid (#E7908, Sigma-Aldrich) using the following primers: FLAG – rat Arsl Fw: 5’ CCCAAGCTTGGGATGGCTCCGCCC 3’; FLAG – rat Arsl Rev:3’ CGGGATCCGCTTCCGGCTCCCGCC 5’. .. The vector was digested using HindIII and BamHI (NEB) restriction enzymes and treated with 5 μl of CIP (alkaline phosphatase, NEB), followed by ligation using the Quick Ligation Kit. ..

    Polymerase Chain Reaction:

    Article Title: Disrupting SOX2 self-association and condensate formation to overcome chemotherapeutic drug resistance in lung squamous cell carcinoma
    Article Snippet: .. The resulting PCR products were recombined into the pHAGE-3 × Flag vector, which was digested with BamHI (NEB, R3136V) and AgeI (NEB, R3552L) via homologous recombination (YEASEN, 10922ES20). .. Similarly, the 6 × His-tagged Helix 1–3, Flag-SOX2, and HA-SOX2 fragments were amplified and recombined into the pSin-Flag vector digested with BamHI and EcoRI (NEB, R3101V).

    Homologous Recombination:

    Article Title: Disrupting SOX2 self-association and condensate formation to overcome chemotherapeutic drug resistance in lung squamous cell carcinoma
    Article Snippet: .. The resulting PCR products were recombined into the pHAGE-3 × Flag vector, which was digested with BamHI (NEB, R3136V) and AgeI (NEB, R3552L) via homologous recombination (YEASEN, 10922ES20). .. Similarly, the 6 × His-tagged Helix 1–3, Flag-SOX2, and HA-SOX2 fragments were amplified and recombined into the pSin-Flag vector digested with BamHI and EcoRI (NEB, R3101V).

    Cloning:

    Article Title: Enhanced Large-Scale Production and Purification of Recombinant Human NUPR1 for Detailed Structural and Functional Insights.
    Article Snippet: .. Restriction enzymes NcoI (5′-C^CATGG-3′) and BamHI (5′-G^GATCC3′) (New England Biolabs) were utilized for directional cloning into the pET28a(+) vector. ..

    Gel Purification:

    Article Title: Hemin activates interleukin-17 signaling and CCAAT/enhancer-binding protein beta to promote neuroinflammation and blood-brain barrier disruption.
    Article Snippet: Heme overload, a feature of hemolytic conditions and cellular injury, can initiate oxidative stress and inflammatory cascades that perturb neurovascular homeostasis.. However, the molecular mechanisms by which peripheral heme exposure disrupts blood–brain barrier (BBB) integrity remain incompletely defined.. Here, we investigated key molecular mediators linking peripheral hemin challenge to neurovascular injury.



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